Digoxin Immune Fab (Ovine)
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چکیده
Digibind, digoxin immune Fab (ovine), is a sterile lyophilized powder of antigen binding fragments (Fab) derived from specific antidigoxin antibodies raised in sheep. Production of antibodies specific for digoxin involves conjugation of digoxin as a hapten to human albumin. Sheep are immunized with this material to produce antibodies specific for the antigenic determinants of the digoxin molecule. The antibody is then papain-digested and digoxin-specific Fab fragments of the antibody are isolated and purified by affinity chromatography. These antibody fragments have a molecular weight of approximately 46,200.
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Effects of sheep digoxin-specific antibodies and their Fab fragments on digoxin pharmacokinetics in dogs.
Intact sheep antidigoxin antibodies and their Fab fragments have both been found to exert profound effects on digoxin pharmacokinetics in [3H] digoxin-treated dogs. Both classes of molecule remove digoxin from the extravascular space and sequester it in the circulation in protein-bound form, a form in which the digoxin is presumably inactive. These two classes of molecule differ, however, in th...
متن کاملAnti-digoxin Fab fragments in cardiotoxicity induced by ingestion of yellow oleander: a randomised controlled trial.
BACKGROUND Severe cardiac glycoside cardiotoxicity after ingestion of yellow oleander seeds is an important problem in rural areas of Sri Lanka. Currently, patients must be transferred to the capital for temporary cardiac pacing. We did a randomised controlled trial to investigate whether anti-digoxin Fab could reverse serious oleander-induced arrhythmias. METHODS After a preliminary dose-fin...
متن کاملMonitoring of unbound digoxin in patients treated with anti-digoxin antigen-binding fragments: a model for the future?
Digoxin is one of the few therapeutic drugs for which antidotal therapy is available (1). Administration of Fab fragments has successfully been used to reverse the effects of life-threatening digoxin overdoses for over 20 years. Digoxin-specific Fab fragments are produced by cleaving sheep digoxin-specific IgG with papain to form two Fab fragments (50 000 Da each) and one 50 000-Da Fc fragment ...
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direct analysis without pretreatment of serum samples. Clin Chem 1998; 44:1947–50. 17. Ward SB, Sjostrom L, Ujhelyi MR. Comparison of the pharmacokinetics and in vivo bioaffinity of Digitab versus Digibind. Ther Drug Monit 2000;22:599– 607. 18. Allen G. The affinity of binding of digoxin to ovine anti-digoxin Fab (Digibind) preparations. Biologicals 1996;24:19–24. 19. Ujhelyi MR, Green PJ, Cumm...
متن کاملAnalysis of the use of digoxin immune fab for the treatment of non-life-threatening digoxin toxicity.
BACKGROUND Studies indicate that digoxin toxicity often results in lengthy hospitalizations and considerable costs, both of which may be decreased through the routine use of digoxin immune Fab (FAB). METHODS A computer-based decision analysis model was developed to compare the treatment of non-life-threatening digoxin toxicity with either FAB or standard therapy from the hospital perspective....
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